Barcelona, Spain – Oct. 17-20, 2011 — At this meeting, results were reported for clinical studies carried out under FDOC/SRD sponsored research by the French National Institute for Agronomic Research (INRA).C. Morand et.al (Oral presentation): ”What role for citrus flavanone in cardiovascular health?”
Summary — For this clinical randomized controlled cross-over study 24 healthy men consumed during 3 one-month periods either 500ml orange juice (OJ), 500ml isoenergetic control drink (CO) or 500 ml control drink with 292mg hesperidin in capsules (HE). Endothelial microvascular reactivity, blood pressure, systemic markers linked to CVD risk and transcriptome of PBMCs were measured at the end of each period. To further validate and increase understanding of cellular and molecular mechanisms revealed by transcriptome studies, the research evaluated the impact of flavanones metabolites used at low doses on the activity and gene expression of endothelial cells.
The authors reported that, in humans, a 4-week consumption of orange juice or hesperidin significantly reduced diastolic blood pressure and improved postprandial microvascular endothelial reactivity. Both orange juice and hesperidin interventions significantly affected leukocyte gene expression (over 1,000 genes differentially expressed) implicated in chemotaxis, adhesion, infiltration and lipid accumulation, and these processes are involved in initial steps of atherosclerosis development.
The researchers suggested their data shows a beneficial impact of citrus flavanone on vascular health and that this latter could be mediated by genomic effects.
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Also Presented at the 5th International Conference on Polyphenols and Health (ICPH)
Results were reported for clinical studies carried out under FDOC/SRD sponsored research by the French National Institute for Agronomic Research (INRA). “M. Quintana, et.al. (Poster presentation) : “Effects of consumption of hesperidin or orange juice on the plasma and urine metabolomic profiles”
Summary — The effects of hesperidin and orange juice on clinical endpoints related to cardiovascular health have been studied in a cross-over controlled study. Healthy men daily consumed for one month either 500ml orange juice (OJ), 500ml isoenergetic control drink (CO) or 500 ml control drink with 292mg hesperidin in capsules (HE). Metabolomic profiles (metabolomes) for 12 volunteers were compared for urine and plasma samples collected at the end of the 3 experimental periods. Samples were analyzed using high resolution mass spectrometry. Component markers were evaluated within samples as potential discriminators for consumption of OJ and its components.
Urine metabolomes were significantly different after consumption of orange juice compared to a control drink. About 100 discriminatory markers were found to be more intense in the OJ group. Most markers are phytochemical metabolites but new potential bioactives may be identified. On the other hand, no differences were observed in the urine metabolomes of subjects from the HE group compared to CO group, except for the expected presence of hesperetin and its main metabolite, hesperetin glucuronide.
In contrast to urine metabolomes, plasma metabolomes were markedly different after the same HE and CO experimental periods, with 70 markers discriminating the metabolomic profiles. This suggests that hesperidin had an impact on the endogenous metabolism. Marked changes have also been observed in the plasma metabolome after OJ consumption. Some modulated markers were identical to those affected by hesperidin, whereas others were only modified with orange juice. In contrast to urine, a large majority of discriminatory ions were endogenous metabolites.
In conclusion, authors reported that metabolomics analysis was able to capture the multiple changes that occurred in biological fluids after one-month consumption of hesperidin or orange juice. Identification of the modulated metabolites still requires substantial work but is likely to provide crucial information on the metabolic targets of hesperidin and orange juice.
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Also Presented at the 5th International Conference on Polyphenols and Health (ICPH)
Dragan Milenkovic of the Human Nutrition Unit of the French National Institute for Agronomic Research (INRA) gave a talk on ” Modulation of miRNA Expression by Polyhenols: Potential New Molecular Targets Underlying Their Health Effects”. Included were data derived from studies on hesperidin utilized within clinical studies carried out under FDOC/SRD sponsored research. This talk included in depth evaluations of polyphenols and their role in modifying genetic expression beneficial to human health.
